Detection of Hammondia heydorni–Like Organisms and Their Differentiation From Neospora caninum Using Random-Amplified Polymorphic DNA–Polymerase Chain Reaction
نویسندگان
چکیده
منابع مشابه
Studies on serological cross-reaction of Neospora caninum with Toxoplasma gondii and Hammondia heydorni.
The enzyme-linked immunosorbent assay (ELISA) was used to examine cross-reactivity of Neospora caninum with Toxoplasma gondii and Hammondia heydorni. Anti-T. gondii mouse and cat sera cross-reacted with N. caninum soluble antigen (NLA), but not with the recombinant surface antigen (NcSRS2). Anti-H. heydorni dog sera showed no cross-reactivity with either the NLA antigen or the NcSRS2. Lack of c...
متن کاملDifferential detection of Hammondia hammondi from Toxoplasma gondii using polymerase chain reaction.
Hammondia hammondi and Toxoplasma gondii are two related coccidian parasites, with cats as definitive hosts and warm-blooded animals as intermediate hosts. It is difficult to differentiate them by morphological and serological parameters. In the present study, primers were designed to specifically amplify the ITS-1 region of H. hammondi to differentiate it from T. gondii. Attempts were made to ...
متن کاملToxoplasma gondii, Neospora caninum, Hammondia hammondi and Hammondia heydorni are closely related coccidian parasites with similarly sized oocysts
hammondi and Hammondia heydorni are closely related coccidian parasites with similarly sized oocysts [1]. Cats (felids) are definitive hosts for T. gondii and H. hammondi, and dogs (canids) are definitive hosts for N. caninum and H. heydorni. However, these species have important biological and ultrastructural differences. Whereas T. gondii causes a disseminated infection in humans and animals,...
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Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...
متن کاملMolecular detection of Neospora caninum from naturally infected dogs in Lorestan province, West of Iran
Neospora caninum is a coccidian protozoan that causes abortion in dairy and beef cattle and neurological disorders in dogs and horses. To identify N. caninum oocysts in the dog feces the molecular approaches are known as sensitive methods that specifically detect the oocysts. In present study, a polymerase chain reaction (PCR) targeting N. caninum specific Nc5 genomic fragment was performed to ...
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ژورنال
عنوان ژورنال: Journal of Parasitology
سال: 2003
ISSN: 0022-3395,1937-2345
DOI: 10.1645/ge-93r